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Custom Reagent Builder

Transformation Buffers

Transformation buffers are specifically formulated to enhance the uptake of foreign DNA by bacterial cells, increasing the success rate of cloning and genetic engineering workflows. These buffers gently destabilize the cell membrane, making it more permeable to plasmids or other DNA constructs.

  • Facilitates high-efficiency DNA uptake in competent cells like E. coli
  • Contains calcium chloride and other ions to promote membrane permeability
  • Ideal for routine cloning, plasmid prep, and genetic manipulation protocols

Whether you're preparing chemically competent cells or optimizing transformation efficiency, our transformation buffers offer dependable performance for molecular biology success.

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Frequently Asked Questions

Transformation buffers are used to prepare bacterial cells, typically E. coli, for uptake of foreign DNA, such as plasmids, during molecular cloning or genetic engineering procedures.

Common ingredients include calcium chloride or magnesium chloride to permeabilize the cell membrane, and glycerol to stabilize cells during cold incubation and freezing.

Most work well with lab strains of E. coli, but optimization may be needed for other species or strains with lower transformation efficiencies.