Stripping buffer enables the reuse of blotting membranes by removing bound antibodies or probes without damaging the immobilized proteins. This allows for multiple rounds of probing on the same membrane, saving time, reducing sample consumption, and improving experimental efficiency.
Effectively removes antibodies from western blots while preserving target proteins
Allows membranes to be reprobed for multiple targets or controls
Available in gentle and harsh formulations to suit different antibodies and membrane types
Whether you're performing sequential western blotting or optimizing antibody panels, our stripping buffer delivers reliable performance without compromising membrane integrity.
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Frequently Asked Questions
What is a stripping buffer used for?
Stripping buffer is used in western blotting and other blot-based assays to remove previously bound antibodies, allowing the membrane to be reprobed with different antibodies.
Can I use any stripping buffer with all membranes?
Buffer selection depends on the membrane type and antibody sensitivity. Some stripping buffers are gentle enough for PVDF or nitrocellulose, while others are more aggressive and may require caution.
Will stripping damage my target protein?
Excessive stripping or harsh conditions can affect the immobilized proteins. Choosing the right formulation and minimizing stripping time helps preserve membrane-bound targets.
