Sample buffer is a critical reagent used to prepare samples for electrophoresis. It ensures proper denaturation, uniform charge distribution, and accurate tracking of migration through the gel, all while protecting the integrity of your sample during loading and separation.
Denatures proteins and normalizes charge for consistent electrophoretic mobility
Available in reducing and non-reducing formats to suit a range of applications
Contains tracking dye for visual monitoring of sample migration through the gel
Whether you're running SDS-PAGE or native gels, our sample buffer delivers the reliable sample preparation you need for clear, reproducible results.
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Frequently Asked Questions
What is a sample buffer used for in electrophoresis?
Sample buffer prepares samples for gel electrophoresis by denaturing the proteins, equalizing charge, and enabling visualization during gel runs.
What’s the difference between reducing and non-reducing sample buffers?
Reducing sample buffers contain agents like DTT or β-mercaptoethanol to break disulfide bonds, which helps fully linearize proteins. Non-reducing buffers leave these bonds intact for detecting multimeric or native forms.
Can sample buffer affect the accuracy of results?
Yes. Using the wrong buffer type (e.g., reducing vs. non-reducing) or loading without buffer can lead to incomplete denaturation, inconsistent banding, or misinterpretation of protein size or structure.
