Blocking buffers are essential for reducing background and improving specificity in western blotting and other membrane-based detection assays. These buffers coat unbound areas of the membrane to prevent non-specific antibody binding, allowing your target signal to stand out clearly and reliably.
Minimizes background by preventing non-specific interactions on membranes
Enhances antibody binding specificity for improved signal clarity
Buffer selection can be optimized based on membrane type and antibody characteristics to improve specificity and reduce background
Whether you're optimizing a western blot or fine-tuning a nucleic acid detection assay, our blocking buffers help ensure clean, reliable results every time.
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Frequently Asked Questions
What are common components of blocking buffers?
Blocking buffers often contain proteins like BSA, non-fat dry milk, or casein, as well as detergents like Tween-20 to cover unoccupied sites on the membrane surface.
How long should I block the membrane?
Typical blocking times range from 30 minutes to 1 hour at room temperature, but some protocols may call for longer or overnight blocking at 4°C for improved results.
Can blocking buffers be reused?
Single-use is recommended for consistent results, especially in sensitive assays. Reusing buffers can introduce background variability or contamination.
