HPLC/FPLC buffers are precisely formulated to support high-performance liquid chromatography and fast protein liquid chromatography workflows. These buffers maintain consistent pH and ionic strength, helping to ensure reproducibility, protect column integrity, and support the selective retention and elution of your analytes.
Designed to reduce variability and improve separation reproducibility
Filtered and pH-balanced to extend column life and reduce system maintenance
Available in formulations suitable for protein purification, small molecule separation, and more
Whether you’re performing method development or routine purification, our HPLC/FPLC buffers provide reliable, application-ready solutions for your chromatography workflows.
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Frequently Asked Questions
What are HPLC/FPLC buffers used for?
These buffers are used in liquid chromatography workflows to maintain pH, ionic strength, and solubility of analytes during separation, ensuring consistent and high-resolution results.
How do I choose between different HPLC/FPLC buffer systems?
Buffer choice depends on the analyte, column chemistry, pH range, and detection method. For example, phosphate-based buffers are common for protein work, while volatile buffers may be preferred for mass spectrometry.
Why is buffer quality important in chromatography?
High-quality buffers help reduce column fouling, improve peak shape, and maintain system pressure, all of which contribute to better reproducibility and column longevity.
