Transfer buffer ensures efficient and uniform migration of proteins or nucleic acids from gels to membranes during blotting procedures like Western, Southern, and Northern blotting. It maintains consistent pH and ionic strength throughout the transfer process, helping preserve target molecule integrity and maximizing signal clarity.
Provides stable pH and ionic environment for effective blot transfer
Supports protein and nucleic acid transfer from SDS-PAGE, Native PAGE, and agarose gels
Compatible with PVDF and nitrocellulose membranes for western, southern, or northern blotting
Whether you're transferring proteins for antibody detection or nucleic acids for hybridization, our transfer buffer delivers reliable performance and sharp, reproducible results.
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Frequently Asked Questions
What are the typical components of a transfer buffer?
Most transfer buffers include Tris (for pH control), glycine (for ionic strength), and optionally methanol, which improves protein binding to membranes and removes residual SDS.
Can transfer buffer be used for both proteins and nucleic acids?
Yes. While the composition may vary slightly, transfer buffers can support both protein and nucleic acid transfer in techniques like Western, Southern, and Northern blotting.
How does transfer buffer differ between SDS-PAGE and Native PAGE?
Buffers for SDS-PAGE often include methanol and SDS to support denatured protein transfer, while native PAGE buffers avoid harsh detergents to preserve protein structure.
