Bis-Tris Transfer Buffer (20X)

The 20X Bis-Tris Transfer Buffer is often used to transfer proteins from Bis-Tris and Tris-Acetate gels to nitrocellulose or PVDF membranes for Western blotting. It is useful as it maintains a neutral environmental pH during transfer protocols, which is suitable for most protein types, crucial for structural preservation of proteins, and effective at minimizing protein denaturation.