Bis-Tris Buffer (0.2 M, pH 6.5)

The Bis-Tris Buffer is a zwitterionic agent commonly used in biological applications. It maintains a pH within 5.8 to 7.2, ideal for biological processes that require stable environments within this range. It is particularly effective in applications such as protein and nucleic acid purification, biochemical assays, and cell culture. The buffering capacity of Bis-Tris is influenced by its concentration, while its pH can be adjusted based on the ratio of Bis-Tris to its conjugate acid or base.