Boston BioProducts' Buffer Calculator

Biological buffers play a key role in maintaining the pH and environmental conditions of scientific processes and ensure controlled experiments with preserved results. The concentration of a buffer's raw materials holds a direct impact in its final application and must have correct measurements.

This calculator is a useful tool to compute buffer concentration in molarity (M, mM, or nM) and prepare buffer solutions using the molar mass of the raw material selected and the desired final volume (L, mL, or µL). The following formulas, including the Henderson-Hasselbach equation ^[1], percentage by weight (w/w) ^[2], and mass by calculation are built in - bypassing any chances of error in your buffer calculations.

How to use the Buffer Calculator:

Select your desired raw material.
Insert the desired final volume and concentration of your buffer into the corresponding fields.
The correct mass of your desired raw material will instantly populate the "Mass" field.

Reference Formulas

Henderson-Hasselbach equation:

pH = pKa + log([A-]/[HA])

Percentage by weight (w/v) =

(1g solute / 100g of solution) × 100

Mass by concentration =

Desired concentration × molar mass of raw material × final volume

Buffer Parameters

Buffer *

Total Volume *Target Concentration *

CAS #:-

pKa:-

Buffer pH Range:-

Formula Weight (g/mol):-

0.00g

Mass:

Need a custom buffer fast?

References

Po, H. N., & Senozan, N. M. (2001). The Henderson-Hasselbalch equation: Its history and limitations. Journal of Chemical Education, 78(11), 1499-1503.
Soult, A. (2020, October 27). Concentrations of solutions. Chemistry for Allied Health. 8.1: Concentrations of Solutions - Chemistry LibreTexts.