Tris Buffered Saline-Tween, TBST (20X, with 2% Tween-20, pH 7.4)

This 20X TBST formulation incorporates 2% Tween‑20 for higher detergent content, ideal for protocols demanding stronger washing or lower background signals. Maintaining a stable pH range of 7.4–8.0, it ensures efficient removal of unbound reagents without disturbing antigen‑antibody complexes. Frequently used as a wash, blocking, or diluent buffer in Western blotting and immunohistochemistry, the concentrated stock allows flexible adjustment of detergent concentration for different assay requirements.