Tris Buffered Saline-Tween, TBST (10X, with 1% Tween-20, pH 7.4)

This 10X TBST buffer is prepared with 1% Tween‑20 for greater detergent activity, making it suitable for more stringent washing conditions in immunochemistry. It effectively removes unbound antibodies and proteins while maintaining stable pH between 7.4 and 8.0. TBST is broadly used in Western blot and ELISA protocols to achieve high‑contrast signal detection with minimal background interference. Its balanced Tris‑saline composition provides optimal conductivity and buffer capacity for repeated assays.