Separating (Resolving) Buffer

CAT#: BP-90

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Need a Custom Formulation?

Modify concentration, pH, packaging, or quality grade.

We manufacture to your exact specifications.

Custom Reagent Builder

Product Description

The Separating (Resolving) Buffer is a critical component in SDS-Polyacrylamide Gel Electrophoresis (PAGE), a laboratory technique that is used to separate protein molecules based on molecular weight. This buffer maintains the pH of the separating or resolving gel at 8.8, which is a higher pH level than that of the stacking gel.

Component Name

CAS

Molecular Weight

Concentration

Tris-Base / Tris-HCl

77-86-1 / 1185-53-1

121.14 / 157.60

1.5 M

Sodium Dodecyl Sulfate (SDS) [20% Solution]

151-21-3

288.38

0.40%

Water

7732-18-5

18.02

Rest

Separating (Resolving) Buffer

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

Need a specific formulation?

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

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Documents

Product Citations

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Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Need a specific formulation?

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Separating (Resolving) Buffer

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

Related Products

Related Resources

Need a specific formulation?

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

Related Products

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Related Resources

Need a specific formulation?

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)