Tris-HCl Buffer (1.5 M, pH 8.8)

1.5 M Tris-HCl Buffer is a concentrated buffer solution composed of tris-HCl and tris base forming a reliable buffering system. This buffer is pH-adjusted to 8.8 for applications requiring a basic pH, such as protein or enzymatic reactions. 1.5 M Tris-HCl is a common buffer used in the resolving or separating gel in polyacrylamide gel electrophoresis (PAGE). The higher pH creates optimal conditions for clean separation when migrating through the gel.