Tris Buffered Saline, TBS (10X with 1% Triton X-100)

TBS (Tris-buffered saline) buffer with Triton is commonly used in biochemical and molecular biology applications, particularly for immunoassays and Western blotting. TBS consists of Tris-HCl and NaCl, maintaining a stable pH, typically around 7.4. The addition of Triton X-100, a non-ionic surfactant, enhances permeability by disrupting lipid membranes, making it useful for cell lysis, protein extraction, and immunostaining. This buffer helps in washing steps by reducing non-specific binding and improving antibody-antigen interactions.