Tricine Buffer (0.2 M, pH 8.0)

This 0.2 M Tricine [N‑tris(hydroxymethyl)methylglycine] buffer is formulated to maintain an alkaline environment at pH 8.0, within its 7.4–8.8 effective range. As a member of the Tris family of buffers, it offers excellent stability and minimal interference with biochemical reactions. Tricine buffer is commonly chosen for peptide electrophoresis in SDS‑PAGE, especially for low‑ to medium‑molecular‑weight proteins. It is also ideal for analytical applications such as capillary electrophoresis, high‑performance liquid chromatography, and ion‑exchange separations, as well as for gentle suspension of cells and biomolecules.