Plasmid DNA Isolation Buffer (pH 7.0)

Plasmid DNA Isolation Buffer is a MOPS-buffered saline solution with Triton X-100 detergent and isopropanol. The buffer is intended for isolation and column equilibration to prepare for DNA binding. MOPS provides buffering capacity, isopropanol assists in establishing correct resin conditions, and Triton minimizes contamination and helps with endotoxin removal. A high concentration of sodium chloride functions as an electrostatic interaction regulator between pDNA and anion-exchange resin.