MES Buffer (0.5 M, pH 6.0)

A 0.5 M MES [2-(N-morpholino)ethanesulfonic acid] buffer specifically formulated for pH 6.0, providing strong buffering capacity in the range of 5.5–7.0. Known for its high solubility and zwitterionic properties, MES is frequently used in protein separation workflows, such as bis-tris electrophoresis or ion-exchange chromatography. It resists metal ion interactions and UV absorption, helping maintain stable pH conditions during enzyme assays and protein structural studies.