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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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Low Salt Lysis
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Low Salt Lysis

Low salt lysis buffer is specially formulated for gentle yet effective cell disruption, particularly from primary cells, tissue samples, or sensitive cultured cells. With reduced ionic strength and built-in inhibitors, it enables extraction of intact proteins suitable for downstream analysis.

  • Preserves protein integrity and phosphorylation with reduced salt-induced denaturation
  • Ideal for lysing delicate cell types without compromising yield or function
  • Compatible with western blotting, ELISA, IP, PAGE, and protein purification workflows

Whether you're studying native protein interactions or preparing lysates for high-sensitivity detection, our low salt lysis buffer gives you clean results without harsh conditions.

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Low Salt Lysis

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Low Salt Lysis Buffer

Low Salt Lysis Buffer

From: $78.00

FAQs

Low salt lysis buffer is used to gently lyse primary cells, tissues, or sensitive cultured cells while preserving protein structure and post-translational modifications for downstream analysis.

Lower ionic strength reduces the risk of protein denaturation, aggregation, or disruption of protein complexes—making it ideal for studying native interactions or extracting fragile proteins.

It’s suitable for immunoprecipitation, western blotting, ELISA, polyacrylamide gel electrophoresis, and many purification methods.