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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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HEPPS
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  5. HEPPS

HEPPS

HEPPS buffer (EPPS) is a versatile Good’s buffer designed for stable pH control in biological and biochemical research. With an effective pH range of 7.3 to 8.7, HEPPS is widely used in cell culture media, protein transfection, and intracellular targeting, making it an excellent choice for mammalian cell studies and biochemical assays.

  • Precise pH stability supports reproducible experimental conditions
  • Enhances protein delivery for improved transfection efficiency
  • Minimal biomolecular interference ensures reliable assay performance

HEPPS buffer provides exceptional stability in physiological conditions, making it a trusted solution for protein research, intracellular delivery, and advanced cell culture applications.

HEPPS

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HEPPS Buffer (0.2 M, pH 8.0)

HEPPS Buffer (0.2 M, pH 8.0)

From: $82.00

FAQs

HEPPS is commonly used in cell culture media, protein transfection, intracellular targeting, and biochemical assays requiring stable pH in the 7.3–8.7 range.

HEPPS facilitates intracellular targeting and protein delivery into mammalian cells by maintaining an optimal biochemical environment for transfection.

Yes, HEPPS is also known as EPPS buffer. Both names refer to the same zwitterionic Good’s buffer commonly used in cell culture and biochemical research.

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